Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.3K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.0K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autofluorescence spectroscopy and multispectral autofluorescence microscopy for characterization of lupus nephritis in renal tissues.

Scientific reports·2026
Same author

Untrained Position-Encoded Multilayer Perceptron Network for Structured Illumination Microscopy Reconstruction.

Chemical & biomedical imaging·2026
Same author

A Two-Stage, Semi-Supervised Deep Learning Framework for the Detection and Classification of Ambient Pollen using Evanescent Wave Scattering.

Environmental science & technology·2026
Same author

Subacute Pulmonary Embolism Masquerading as Musculoskeletal Thoracic Back Pain: A Case Report.

Pain medicine case reports·2026
Same author

Status of Evidence on the Efficacy and Safety of Indian Traditional Medicine for Prediabetes and Type 2 Diabetes Mellitus: Protocol for a Systematic Review and Evidence Map Synthesis.

JMIR research protocols·2026
Same author

Efficacy of Ayurveda Regimen as an Adjunct to Hydroxyurea in Sickle Cell Disease: Protocol for a Prospective, Randomized, Open-Label, Blinded End Point Exploratory Study.

JMIR research protocols·2026
Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026
Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026
Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026
Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026
Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026
Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Oct 25, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.1K

High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent

Azeem Ahmad1, Vishesh Dubey2, Nikhil Jayakumar2

  • 1Department of Physics and Technology, UiT The Arctic University of Norway, 9037, Tromsø, Norway. ahmadazeem870@gmail.com.

Scientific Reports
|August 5, 2021
PubMed
Summary
This summary is machine-generated.

A novel pseudo-thermal light source (PTLS) enhances quantitative phase microscopy (QPM) by improving spatial phase sensitivity and field of view (FOV). This breakthrough enables high-throughput, minimally invasive biomedical imaging applications.

More Related Videos

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.8K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.2K

Related Experiment Videos

Last Updated: Oct 25, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

7.1K
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.8K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.2K

Area of Science:

  • Optical microscopy
  • Biomedical imaging
  • Phase contrast imaging

Background:

  • Quantitative Phase Microscopy (QPM) requires high space-bandwidth product and spatial phase sensitivity for biomedical applications.
  • Conventional light sources in QPM present trade-offs between temporal resolution, field of view (FOV), and spatial phase sensitivity.
  • Low coherence sources offer high phase sensitivity but limit temporal resolution or FOV, while high coherence sources (lasers) offer large FOV but reduced phase sensitivity.

Purpose of the Study:

  • To demonstrate that a narrowband partially spatially coherent light source, a pseudo-thermal light source (PTLS), overcomes limitations of conventional light sources in single-shot QPM.
  • To compare the performance of PTLS with conventional light sources regarding space-bandwidth product, phase sensitivity, and imaging quality.
  • To showcase the capabilities of PTLS-based QPM for both amplitude and phase objects, including live biological samples.

Main Methods:

  • Implementation of a pseudo-thermal light source (PTLS) in a single-shot quantitative phase microscopy (QPM) system.
  • Comparative analysis of PTLS against conventional low and high coherence light sources.
  • Performance evaluation using USAF resolution charts for amplitude objects and thin optical waveguides for phase objects.
  • Demonstration of high-speed imaging with live sperm cells and large FOV imaging with human placenta tissue samples.

Main Results:

  • PTLS in QPM achieves spatial phase sensitivity equivalent to white light sources.
  • PTLS supports a field of view (FOV) comparable to laser light sources, approximately 18 times larger than low coherence sources.
  • The system demonstrates high-speed imaging capabilities suitable for live cell analysis and large-area tissue imaging.
  • PTLS overcomes the limitations of conventional light sources, offering a superior balance of spatial phase sensitivity and FOV.

Conclusions:

  • Pseudo-thermal light sources (PTLS) significantly enhance single-shot Quantitative Phase Microscopy (QPM) systems.
  • PTLS-based QPM offers a unique combination of high spatial phase sensitivity, large field of view, and high-speed imaging.
  • This advancement facilitates minimally invasive, high-throughput, and spatially sensitive imaging, paving the way for wider adoption in life sciences and clinical diagnostics.