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Fast screening of Depolymerized Lignin Samples Through 2D-Liquid Chromatography Mapping.

Tibo De Saegher1, Jeroen Lauwaert1, Joeri Vercammen1,2

  • 1Department of Materials Textiles and Chemical Engineering (MaTCh), Ghent University, Valentin Vaerwyckweg 1, 9000, Ghent, Belgium.

Chemistryopen
|August 5, 2021
PubMed
Summary

This study introduces a new GPC-HPLC-UV/VIS method for analyzing lignin depolymerization products. The technique effectively separates bio-aromatics by size and polarity, aiding catalyst screening in green chemistry.

Keywords:
analytical methodsbio-aromaticscatalysts developmentlignin depolymerizationtwo dimensional liquid chromatography

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Area of Science:

  • Green chemistry
  • Catalysis
  • Analytical chemistry

Background:

  • Lignin valorization is crucial for sustainable chemical production.
  • Current analytical methods for lignin depolymerization products are limited, hindering catalyst and reaction optimization.
  • Existing techniques often overlook larger oligomeric compounds, focusing mainly on monomers and dimers.

Purpose of the Study:

  • To bridge the analytical gap in lignin depolymerization product analysis.
  • To develop a method for comprehensive mapping of bio-aromatic product pools.
  • To enable faster and more detailed screening of catalysts and reaction conditions.

Main Methods:

  • Development of a quasi-orthogonal Gel Permeation Chromatography-High-Performance Liquid Chromatography-UV/VIS (GPC-HPLC-UV/VIS) method.
  • Separation of bio-aromatics based on molecular weight (hydrodynamic volume) and polarity.
  • Evaluation of the method using model compounds and real lignin depolymerization samples.

Main Results:

  • The developed GPC-HPLC-UV/VIS method successfully separates bio-aromatics by both molecular weight and polarity.
  • Color plots generated by the method provide a rapid graphical assessment of product distribution.
  • The technique effectively differentiates reaction selectivity towards monomers, dimers, and oligomers.

Conclusions:

  • The quasi-orthogonal GPC-HPLC-UV/VIS method addresses a critical analytical gap in lignin valorization.
  • This analytical advancement facilitates efficient screening of catalysts and reaction conditions for bio-aromatic production.
  • The method offers a powerful tool for understanding and optimizing lignin depolymerization processes.