Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.0K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

9.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
9.9K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

18.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
18.3K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

770
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
770

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Extracellular vesicle cargo dynamics in the bone marrow microenvironment: from hematopoietic homeostasis to malignant transformation.

Extracellular vesicle·2026
Same author

Intermittent parathyroid hormone employs autonomous and non-autonomous mechanisms to drive osteogenesis from Ebf3-expressing skeletal progenitor cells.

bioRxiv : the preprint server for biology·2026
Same author

Relationship between asporin expression and the pathological progression of murine temporomandibular joint osteoarthritis after partial discectomy.

Journal of oral biosciences·2026
Same author

LepR<sup>+</sup> skeletal stem/progenitor cells in craniofacial tissues.

Journal of oral biosciences·2026
Same author

Tie2 inhibition disrupts TMEM doorway function and reduces dissemination in pancreatic ductal adenocarcinoma.

Journal of experimental & clinical cancer research : CR·2026
Same author

Sox9 contributes to postnatal opening of the murine parotid papilla.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·2026

Related Experiment Video

Updated: Oct 25, 2025

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
09:00

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Published on: April 22, 2014

19.4K

Intravital fluorescence microscopy with negative contrast.

Juwell W Wu1, Yookyung Jung1,2, Shu-Chi A Yeh1

  • 1Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America.

Plos One
|August 5, 2021
PubMed
Summary
This summary is machine-generated.

A new intravital microscopy method uses fluorescent tracers in extracellular fluid to visualize unlabeled cells, providing better biological context. This technique enhances understanding of cell density, distribution, and dynamics in vivo.

More Related Videos

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
08:52

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Published on: January 19, 2018

14.2K
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
08:23

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Published on: September 1, 2013

23.9K

Related Experiment Videos

Last Updated: Oct 25, 2025

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy
09:00

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Published on: April 22, 2014

19.4K
Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
08:52

Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

Published on: January 19, 2018

14.2K
Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
08:23

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

Published on: September 1, 2013

23.9K

Area of Science:

  • Cell Biology
  • Microscopy
  • In Vivo Imaging

Background:

  • Intravital microscopy (IVM) allows studying cellular dynamics in living organisms.
  • Standard fluorescence labeling in IVM makes unlabeled cells invisible, limiting contextual understanding.
  • Existing methods lack comprehensive visualization of the cellular microenvironment.

Purpose of the Study:

  • To develop a novel method for visualizing unlabeled cells in IVM.
  • To enhance the biological context of in vivo cell imaging.
  • To provide a more complete view of cellular organization and dynamics.

Main Methods:

  • Utilizing extracellular fluid and common vascular/lymphatic tracers for fluorescence labeling.
  • Applying tracers to bone marrow (BM) and lymph node (LN) for IVM.
  • Generating negative contrast images to complement standard positive cell labeling.

Main Results:

  • Fluorescent tracers readily perfuse interstitial spaces, outlining unlabeled cells.
  • The method provides negative contrast images, complementing positive cell labeling.
  • Comprehensive visualization of cellular landscape, density, spatial distribution, and motility.

Conclusions:

  • The novel method effectively "fills the void" in IVM by visualizing unlabeled cells.
  • It offers a simple yet powerful approach for detailed in vivo cellular analysis.
  • Extracellular dye localization and interstitial flow support prolonged, low-toxicity imaging.