Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Comparison between RL and RC circuits01:24

Comparison between RL and RC circuits

4.7K
An RC circuit consists of resistance and capacitance, while in an RL circuit, capacitance is replaced by an inductor. RL and RC circuits are first-order differential circuits that store energy. An RC circuit stores energy in the electric field, while an RL circuit stores energy in the magnetic field. When connected to a battery, an RC circuit charges the capacitor, causing the current to decrease from maximum to zero upon being fully charged. This increases the voltage across the capacitor from...
4.7K
The R Chart01:02

The R Chart

193
In statistical process control, control charts, particularly R charts, are instrumental in monitoring process variations and identifying non-random patterns that run charts might miss. R charts track the variability within process subgroups, which is crucial when standard deviation use is impractical or unknown process variations exist.
R charts are pivotal for pinpointing shifts in process variability. Stability is indicated when all data points remain within the defined upper and lower...
193
Regression Toward the Mean01:52

Regression Toward the Mean

6.6K
Regression toward the mean (“RTM”) is a phenomenon in which extremely high or low values—for example, and individual’s blood pressure at a particular moment—appear closer to a group’s average upon remeasuring. Although this statistical peculiarity is the result of random error and chance, it has been problematic across various medical, scientific, financial and psychological applications. In particular, RTM, if not taken into account, can interfere when...
6.6K
Relative Risk01:12

Relative Risk

587
Relative risk (RR) is a statistical measure commonly used in epidemiology to compare the likelihood of a particular event occurring between two groups. This metric is important for evaluating the relationship between exposure to a specific risk factor and the probability of a particular outcome. It plays a crucial role in medical research, public health studies, and risk assessment. Relative risk quantifies how much more (or less) likely an event is to occur in an exposed group compared to an...
587
Introduction to R01:11

Introduction to R

3.0K
R is a powerful software environment for statistical computing and graphics. Originating as an implementation of the S language, developed at Bell Laboratories, R has evolved into a robust, open-source statistical software favored by statisticians and data scientists worldwide. Its comprehensive suite includes data manipulation, calculation, and graphical display capabilities, making it versatile for data analysis and visualization. Its programming language is at the core of R's...
3.0K
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

15.4K
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
15.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trans-species microRNA loci in the parasitic plant Cuscuta campestris have a U6-like snRNA promoter.

The Plant cell·2023
Same author

Evaluation of Virus-Free and Wild-Type Isolates of <i>Pseudogymnoascus destructans</i> Using a Porcine Ear Model.

mSphere·2022
Same author

Chemical capping improves template switching and enhances sequencing of small RNAs.

Nucleic acids research·2021
Same author

Phylogeographic analysis of Pseudogymnoascus destructans partitivirus-pa explains the spread dynamics of white-nose syndrome in North America.

PLoS pathogens·2021
Same author

A 1,000-Year-Old RNA Virus.

Journal of virology·2018
Same author

Characterizing Mycoviruses.

Methods in molecular biology (Clifton, N.J.)·2018

Related Experiment Video

Updated: Oct 25, 2025

RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans
11:09

RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans

Published on: July 17, 2021

3.2K

RdRp or RT, That is the Question.

Mahtab Peyambari1, Shengxi Guan2, Marilyn J Roossinck1

  • 1Center for Infectious Disease Dynamics, Millennium Science Complex, Pennsylvania State University, University Park, PA, USA.

Molecular Biology and Evolution
|August 5, 2021
PubMed
Summary

Partitivirus RNA-dependent RNA polymerases (RdRps) exhibit reverse transcriptase activity, synthesizing DNA from RNA templates. This suggests dsRNA virus RdRps may be ancestral to other RdRps.

Keywords:
RNA-dependent RNA polymerasepartitiviruspolymerase originsreverse transcriptasevirus evolution

More Related Videos

Three Laboratory Procedures for Assessing Different Manifestations of Impulsivity in Rats
09:12

Three Laboratory Procedures for Assessing Different Manifestations of Impulsivity in Rats

Published on: March 17, 2019

9.6K
Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform RpEGEN for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium
13:12

Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform RpEGEN for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

Published on: March 2, 2022

2.7K

Related Experiment Videos

Last Updated: Oct 25, 2025

RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans
11:09

RBDT: A Computerized Task System based in Transposition for the Continuous Analysis of Relational Behavior Dynamics in Humans

Published on: July 17, 2021

3.2K
Three Laboratory Procedures for Assessing Different Manifestations of Impulsivity in Rats
09:12

Three Laboratory Procedures for Assessing Different Manifestations of Impulsivity in Rats

Published on: March 17, 2019

9.6K
Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform RpEGEN for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium
13:12

Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform RpEGEN for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

Published on: March 2, 2022

2.7K

Area of Science:

  • Virology
  • Molecular Biology
  • Evolutionary Biology

Background:

  • RNA-dependent RNA polymerase (RdRp) is crucial for double-stranded RNA (dsRNA) virus transcription and replication.
  • The location and function of RdRp within the viral particle are key to viral life cycles.

Purpose of the Study:

  • To investigate the enzymatic functions of partitivirus RNA-dependent RNA polymerase (RdRp).
  • To determine if partitivirus RdRps possess reverse transcriptase (RT) activity.
  • To explore the evolutionary implications of RdRp function in dsRNA viruses.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) assays were employed.
  • Purified partitivirus virions and recombinant RdRp proteins were analyzed.
  • Enzymatic activity was tested using various RNA templates (ssRNA, dsRNA) and temperatures.

Main Results:

  • Partitivirus RdRps demonstrated significant reverse transcriptase (RT) function, synthesizing DNA from dsRNA templates.
  • The RdRp activity was observed with both homologous and heterologous dsRNA, as well as ssRNA templates.
  • Partitivirus RdRps were active at lower temperatures than commercial RT enzymes, unlike poliovirus 3Dpol.

Conclusions:

  • Partitivirus RdRps possess dual RNA polymerase and reverse transcriptase activities.
  • This finding challenges the traditional understanding of RdRp functions in dsRNA viruses.
  • A novel evolutionary model is proposed where dsRNA virus RdRps could be ancestral to other RdRps.