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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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THz-enhanced dynamic nuclear polarized liquid spectrometer.

Zhekai Zhang1, Yi Jiang2, Haiya Pi1

  • 1State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|August 5, 2021
PubMed
Summary

This study developed a self-constructed 5 Tesla Dynamic Nuclear Polarization (DNP) spectrometer for liquid samples. The novel spectrometer achieved over 100-fold signal enhancement for Carbon-13 Nuclear Magnetic Resonance (NMR) spectroscopy.

Keywords:
DNP spectrometerDynamic nuclear polarization (DNP)Microwave sourceNuclear magnetic resonance (NMR)

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Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Dynamic Nuclear Polarization (DNP)
  • Spectrometer Development

Background:

  • Dynamic Nuclear Polarization (DNP) significantly enhances NMR signal detection.
  • High-field NMR requires sensitive detection methods for broader applications.

Purpose of the Study:

  • To develop a self-constructed 5 Tesla DNP spectrometer for liquid samples.
  • To evaluate the 13C DNP enhancement capabilities of the developed system.

Main Methods:

  • Construction of a 5 T DNP spectrometer including a wide-bore superconducting magnet, custom console, and dual resonance probe.
  • Development of a 140 GHz microwave source utilizing a traveling wave tube (TWT) amplifier with 4.4 W output power and 1 GHz tuning range.
  • Testing the spectrometer with a liquid 13CCl4 sample to measure 13C NMR signal enhancement.

Main Results:

  • The self-constructed liquid-state DNP spectrometer was successfully developed.
  • A 13C DNP enhancement exceeding 100-fold was observed for the 13CCl4 sample.
  • The performance validates the effectiveness of DNP in liquid-state high-field NMR.

Conclusions:

  • The developed 5 T DNP spectrometer demonstrates superior performance for liquid samples.
  • DNP technology offers significant advantages for enhancing sensitivity in high-field NMR.
  • This work contributes to the advancement of DNP-enhanced NMR instrumentation.