Mitochondria-targeted photoacoustic probe for imaging of hydrogen peroxide in inflamed mouse model

In this chapter, we gave a brief introduction of hydrogen peroxide (H₂O₂) and its existing analytical methods and described the need of mitochondria-targeted photoacoustic (PA) probe for H₂O₂ detection in vivo. Then we provided the detailed protocols for the design and characterization of a mitochondria-targeted PA probe (TPP-HCy-BOH) to visualize H₂O₂in vivo, which was developed in our previous work. Compared to control probe without mitochondria-targeted ability (HCy-BOH), TPP-HCy-BOH could efficiently accumulate in mitochondria and activate its PA signals toward overproduced H₂O₂ in inflamed mouse model with higher PA sensitivity.