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Updated: Oct 25, 2025
Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
A general strategy to optimize the performance of aza-BODIPY-based probes for enhanced photoacoustic properties
Anuj K Yadav1, Rodrigo Tapia Hernandez1, Jefferson Chan1
1Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, United States.
Abstract:
In this chapter, we describe a generalizable strategy to obtain a high PA output platform that is optimized for ratiometric imaging. Our approach entails conformationally restricting pendant aryl rings on the aza-BODIPY core to enhance orbital overlap which consequently increases the extinction coefficient. This strategy can potentially be applied to other dye platforms to enhance their signal intensity. We provide detailed protocols for the synthesis, in vitro characterization, and in vivo application.
