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Lipid production by oleaginous yeasts
Atrayee Chattopadhyay1, Mrinal K Maiti1
1Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, India.
Advances in Applied Microbiology
|August 6, 2021
Summary
This review highlights oleaginous yeasts for sustainable lipid production. Advances in metabolic engineering and genetic tools are accelerating the development of these yeasts as cost-effective, industrial lipid feedstocks.
Area of Science:
- Biotechnology
- Microbiology
- Metabolic Engineering
Background:
- Lipid metabolic engineering in high-lipid-accumulating yeasts is hindered by limited understanding of metabolic pathways and regulatory mechanisms.
- Oleaginous yeasts offer potential for sustainable lipid production due to their high accumulation capabilities.
Purpose of the Study:
- To review prominent oleaginous yeast genera and their characteristics relevant to industrial lipid production.
- To highlight metabolic strategies and genetic tools for enhancing lipid production in yeasts.
Main Methods:
- Curated in silico data on lipid accumulation in oleaginous yeasts.
- Inspection of genetic elements (promoters, terminators, markers) developed for yeast engineering.
- Review of advanced genetic toolboxes and techniques for yeast metabolic engineering.
Main Results:
- Oleaginous yeasts possess desirable traits like cheap carbon source utilization and favorable fatty acid profiles.
- Various strategies and genetic tools have been successfully implemented to improve lipid production.
- Advanced techniques enhance homologous recombination, genome editing, and transformation efficiencies in yeasts.
Conclusions:
- Oleaginous yeasts are promising feedstocks for economically viable lipid production.
- Metabolic engineering and synthetic biology approaches are crucial for developing industrial yeast strains.
- Continued research into yeast metabolism and genetic tools will drive innovation in microbial lipid production.
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