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Updated: Oct 25, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Imaging biological tissue with high-throughput single-pixel compressive holography.

Daixuan Wu^1,2, Jiawei Luo^1,2, Guoqiang Huang^1,2

¹Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China.

Nature Communications

|August 6, 2021

Summary

Single-pixel holography (SPH) achieves high-throughput imaging by using a single-pixel detector. This advanced technique offers scalable resolution and field of view for biophotonics applications.

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Area of Science:

Optics and Photonics
Biophotonics
Computational Imaging

Background:

Single-pixel detectors offer advantages over pixel-array detectors for specific wavelengths due to lower noise, higher sensitivity, and lower cost.
Single-pixel holography (SPH) leverages these detectors for holographic imaging, particularly where conventional detectors are unavailable or expensive.

Purpose of the Study:

To develop a high-throughput single-pixel compressive holography system.
To demonstrate the system's scalability in field of view and resolution.
To image biological tissues with high-resolution amplitude and phase contrast.

Main Methods:

Implemented a compressive holography system utilizing a single-pixel detector.
Achieved high throughput (41,667 pixels/s) by temporal phase stepping, eliminating the need for phase-encoded illumination.
Demonstrated system scalability for large field of view (~83 mm²) or high resolution (5.80 μm × 4.31 μm).

Main Results:

Generated holographic images with rich spatial information using only a single-pixel detector.
Achieved high-throughput imaging with a space-bandwidth-time product of 41,667 pixels/s.
Acquired high-resolution holographic images of biological tissues with excellent amplitude and phase contrast.

Conclusions:

This work presents a significant advancement in single-pixel compressive holography, enabling high-throughput and scalable imaging.
The developed system demonstrates the potential of single-pixel detectors for advanced biophotonics applications, including multi-spectrum imaging.
High-resolution holographic imaging of biological tissues showcases the system's capability for detailed structural analysis.