Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A stopped-flow calorimeter for biochemical applications.

J V Howarth1, N C Millar, H Gutfreund

  • 1Marine Biological Association, Plymouth, U.K.

The Biochemical Journal
|December 15, 1987
PubMed
Summary

A new rapid-response stopped-flow calorimeter is presented for analyzing small reagent samples. This instrument effectively resolves enzyme kinetics, showing potential for chemical and biochemical research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The nature of entropy and its role in biochemical processes.

Advances in enzymology and related subjects of biochemistry·2014
Same author

The sedimentation constant of insulin.

The Biochemical journal·2010
Same author

The identification of chemical intermediates in enzyme catalysis by the rapid quench-flow technique.

Cellular and molecular life sciences : CMLS·2006
Same author

I was lucky, I was there at the right time.

Cellular and molecular life sciences : CMLS·2004
Same author

Rapid-flow techniques and their contributions to enzymology.

Trends in biochemical sciences·1999
Same author

Keith Dalziel: 24 August 1921 - 7 January 1994.

Biographical memoirs of fellows of the Royal Society. Royal Society (Great Britain)·1996

Area of Science:

  • * Calorimetry
  • * Chemical Kinetics
  • * Enzyme Assays

Background:

  • * Traditional calorimetry methods may require larger sample volumes.
  • * Rapid kinetic measurements are crucial for understanding fast biological and chemical processes.
  • * Enzyme-catalyzed reactions often proceed rapidly, necessitating swift analytical techniques.

Purpose of the Study:

  • * To describe the construction and performance of a novel rapid-response stopped-flow calorimeter.
  • * To demonstrate the instrument's capability in resolving complex reaction kinetics.
  • * To highlight the potential applications of this calorimeter in scientific research.

Main Methods:

  • * Design and construction of a stopped-flow apparatus integrated with a calorimeter.
  • * Characterization of the calorimeter's response time and sensitivity.
  • * Application of the calorimeter to study the kinetics of an enzyme-catalyzed hydrolysis reaction.

Main Results:

  • * The stopped-flow calorimeter demonstrated rapid response times suitable for kinetic studies.
  • * The instrument successfully resolved the kinetics of an enzyme-catalyzed hydrolysis.
  • * Performance characteristics and operational limitations were established.

Conclusions:

  • * The developed rapid-response stopped-flow calorimeter is a valuable tool for analyzing small sample volumes.
  • * The method is effective for determining the kinetics of fast reactions, including enzyme-catalyzed processes.
  • * This technique holds significant promise for diverse chemical and biochemical investigations.

Related Experiment Videos