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Fluorimetric determination of levoprotiline in human plasma after thin-layer chromatographic or high performance
C Horne1, H Spahn, E Mutschler
1Pharmakologisches Institut für Naturwissenschaftler, Johann Wolfgang Goethe-Universität, Frankfurt/Main, Fed. Rep. of Germany.
Abstract:
The quantitative determination of the new antidepressant drug levoprotiline, the R-(-)-enantiomer of oxa-protiline (a-[methylamino)methyl)-9,10-ethanoanthracene-9(10H)-ethanol), in human biological material is described. Analysis is performed by alkaline extraction with n-heptane-isopropanol, subsequent fluorescence derivatisation with NBD chloride (4-chloro-7-nitrobenzofurazan), thin layer or high performance liquid chromatography, and fluorimetric measurement of the derivatisation product (lambda max ex. = 470 nm, lambda max em. = 525 nm). The sensitivity of the procedure (detection limit less than 1 ng/ml) permits the performance of pharmacokinetic studies after therapeutic doses.