Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Design for a fast fluorescence laser scanning microscope.

R V Shack1, P H Bartels, R A Buchroeder

  • 1Optical Sciences Center, University of Arizona, Tucson 85721.

Analytical and Quantitative Cytology and Histology
|December 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Final analysis of the ALTTO trial: adjuvant trastuzumab in sequence or in combination with lapatinib in patients with HER2-positive early breast cancer [BIG 2-06/NCCTG N063D (Alliance)].

ESMO open·2024
Same author

N0332 phase 2 trial of weekly irinotecan hydrochloride and docetaxel in refractory metastatic breast cancer: a North Central Cancer Treatment Group (NCCTG) Trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2018
Same author

A prospective genome-wide study of prostate cancer metastases reveals association of wnt pathway activation and increased cell cycle proliferation with primary resistance to abiraterone acetate-prednisone.

Annals of oncology : official journal of the European Society for Medical Oncology·2017
Same author

Differentiation of immortalized epithelial cells derived from cystic fibrosis airway submucosal glands.

In vitro cellular & developmental biology. Animal·2016
Same author

Global acetylation and methylation changes predict papillary urothelial neoplasia of low malignant potential recurrence: a quantitative analysis.

International journal of immunopathology and pharmacology·2011
Same author

y? Diagram, a powerful optical design method for laser systems.

Applied optics·2010
Same journal

Micronuclei and other nuclear anomalies in buccal mucosa following exposure to X-ray radiation.

Analytical and quantitative cytology and histology·2012
Same journal

Color deconvolution. Optimizing handling of 3D unitary optical density vectors with polar coordinates.

Analytical and quantitative cytology and histology·2012
Same journal

Nuclear morphometry in the differential diagnosis of eosinophilic renal epithelial tumors.

Analytical and quantitative cytology and histology·2012
Same journal

Spectrum of prostatic nonepithelial tumors and tumorlike conditions with spindle cell features.

Analytical and quantitative cytology and histology·2012
Same journal

A framework for quantitative assessment of Ki67 distribution in preneoplastic bronchial epithelial lesions.

Analytical and quantitative cytology and histology·2012
Same journal

Classification and grading of noninvasive and invasive neoplasms of the urothelium.

Analytical and quantitative cytology and histology·2012
See all related articles

A new fast fluorescence laser scanning microscope is being developed for high-speed, high-density imaging of large areas. This advanced optical system aims to significantly increase information generation rates compared to current technologies.

Area of Science:

  • Optical microscopy
  • Biophotonics
  • Instrumentation science

Background:

  • Traditional laser scanning microscopy can be limited by scanning speed and data acquisition rates.
  • High-speed imaging is crucial for dynamic biological processes and large-scale sample analysis.

Purpose of the Study:

  • To describe the design and development of a novel fast fluorescence laser scanning microscope.
  • To achieve very-high-speed scanning with high spatial sampling density over large object areas.
  • To create a flexible system applicable to a wide range of research needs.

Main Methods:

  • Detailed design considerations for principal components, including optical elements.
  • Development of a system architecture for rapid data acquisition and processing.

Related Experiment Videos

  • Integration of advanced optical and scanning technologies.

    • Main Results:

    • The system is projected to achieve scanning rates comparable to flow cytometry.
    • Expected information generation rates are orders of magnitude higher than current systems.
    • The design emphasizes flexibility for diverse applications.

    Conclusions:

    • The developed fast fluorescence laser scanning microscope promises significant advancements in imaging speed and data throughput.
    • This instrument is poised to enable new research possibilities in various scientific fields.
    • The project highlights progress in optical instrumentation for high-performance imaging.