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Insight into the AcrAB-TolC Complex Assembly Process Learned from Competition Studies.

Prasangi Rajapaksha1, Isoiza Ojo1, Ling Yang1

  • 1Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA.

Antibiotics (Basel, Switzerland)
|August 6, 2021
PubMed
Summary
This summary is machine-generated.

Overexpressing defective AcrAB-TolC pump components in E. coli did not inhibit multidrug resistance. This suggests a proofreading mechanism prevents the assembly of non-functional bacterial efflux pumps.

Keywords:
RND pumpassemblydominant negative effectmutationprotein-protein interaction

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • The AcrAB-TolC efflux pump complex in Gram-negative bacteria is crucial for multidrug resistance.
  • While its structure is known, the dynamic assembly process is not well understood.

Purpose of the Study:

  • To investigate the assembly mechanism of the AcrAB-TolC efflux pump.
  • To determine if functionally defective components can impede the assembly of functional pump complexes, exhibiting a dominant negative effect.

Main Methods:

  • Overexpression of functionally defective AcrA and AcrB mutants in wild-type E. coli.
  • Analysis of the dominant negative effect on efflux efficiency.

Main Results:

  • No dominant negative effect was observed even with high expression levels of defective pump components.
  • The assembly of the AcrAB-TolC complex appears to have a robust proofreading mechanism.

Conclusions:

  • The E. coli AcrAB-TolC pump assembly process effectively prevents the formation of non-functional complexes.
  • A proofreading mechanism ensures the integrity and functionality of the multidrug efflux pump system.