Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A hemolytic plate method for alternative-pathway complement activity assay.

J S Fong, L Renaud

    American Journal of Clinical Pathology
    |February 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    One-Day Brentuximab-Bendamustine (120 mg/m2) Every 21 Days is a Feasible, Safe and Effective Treatment for Relapsed/Refractory Hodgkin Lymphoma.

    Hematological oncology·2024
    Same author

    STochastic Optical Reconstruction Microscopy (STORM) reveals the nanoscale organization of pathological aggregates in human brain.

    Neuropathology and applied neurobiology·2020
    Same author

    Polarization mechanisms of dielectric materials at a binary liquid interface: impacts on electrowetting actuation.

    Physical chemistry chemical physics : PCCP·2017
    Same author

    Synchrotron X-ray microtransections: a non invasive approach for epileptic seizures arising from eloquent cortical areas.

    Scientific reports·2016
    Same author

    An aptasensor for ochratoxin A based on grafting of polyethylene glycol on a boron-doped diamond microcell.

    Analytical biochemistry·2015
    Same author

    [Pomalidomide for multiple myeloma].

    La Revue de medecine interne·2015
    Same journal

    Invasive urothelial carcinoma in association with surface low-grade papillary urothelial carcinoma: clinical and pathologic insights from a rare entity.

    American journal of clinical pathology·2026
    Same journal

    CEACAM1 expression by immunohistochemistry in B-cell lymphomas and plasma cell myeloma.

    American journal of clinical pathology·2026
    Same journal

    Comprehensive multicriteria life cycle assessment of biopsy processing in a surgical pathology department.

    American journal of clinical pathology·2026
    Same journal

    Prognostic significance of Myb protein and its downstream target genes in lacrimal gland adenoid cystic carcinoma.

    American journal of clinical pathology·2026
    Same journal

    Mismatch repair protein "nonclassic expression loss" pattern in colorectal cancer: an important staining pattern that is not well understood.

    American journal of clinical pathology·2026
    Same journal

    Musculoskeletal pain in medical laboratory personnel: a cross-sectional study.

    American journal of clinical pathology·2026
    See all related articles

    A novel hemolytic plate assay simplifies the assessment of alternative-pathway complement activity. This easy-to-perform method provides reliable functional complement testing for clinical and research applications.

    Area of Science:

    • Immunology
    • Complement System
    • Assay Development

    Background:

    • The complement system is crucial for innate and adaptive immunity.
    • Functional assessment of complement pathways is vital for diagnosing and managing immune disorders.
    • Existing methods for alternative pathway assessment can be complex or time-consuming.

    Purpose of the Study:

    • To describe a new, user-friendly hemolytic plate method for assessing alternative-pathway complement activity.
    • To establish the feasibility and reproducibility of this novel assay.

    Main Methods:

    • Development of a novel hemolytic plate assay.
    • Preparation of specialized plates for complement activity assessment.
    • Functional testing using serum samples.

    Related Experiment Videos

    Main Results:

    • The described hemolytic plate method is easy to prepare and simple to perform.
    • Analysis of 23 normal serum samples yielded a mean lysis diameter of 4.1 ± 0.2 mm.
    • This indicates a reliable and reproducible measure of alternative-pathway complement function.

    Conclusions:

    • The new hemolytic plate method offers a straightforward approach for functional assessment of alternative-pathway complement activity.
    • This assay is suitable for both research and potential clinical diagnostic applications.
    • Further validation in diverse patient populations is warranted.