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Related Concept Videos

Imaging Studies for Cardiovascular System V: CT01:28

Imaging Studies for Cardiovascular System V: CT

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Cardiac computed tomography (CT) scanning is an advanced cardiac imaging technique that utilizes CT technology, with or without intravenous (IV) contrast, to produce accurate cross-sectional virtual slices of specific areas of the heart, coronary circulation, and major blood vessels such as the aorta, pulmonary veins, and arteries. The computer processes these slices to generate three-dimensional images. Multidetector CT (MDCT) is a rapid form of CT scanning that captures multiple slices...
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Imaging Studies for Cardiovascular System IV: CMRI01:21

Imaging Studies for Cardiovascular System IV: CMRI

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Cardiovascular magnetic resonance imaging, or CMRI, is a non-invasive diagnostic test that employs a magnetic field and radiofrequency waves to create precise images of the heart and arteries. It provides comprehensive information about cardiac anatomy, function, perfusion, and tissue characterization without ionizing radiation.IndicationsCMRI diagnoses various heart conditions, including tissue damage from heart attacks, ischemic heart disease, myocarditis, aortic issues (tears, aneurysms,...
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Imaging Studies for Cardiovascular System III: X-Ray01:20

Imaging Studies for Cardiovascular System III: X-Ray

328
The most common cardiovascular diagnostic test is an X-ray. It produces images of the heart, blood vessels, and adjacent structures.
Definition and Purpose
An X-ray, or radiograph, is a non-invasive method that uses ionizing radiation to take images of internal structures. It is mainly used in cardiac imaging to examine the heart, lungs, and major blood vessels, aiming to identify abnormalities in the heart's size, shape, and position, such as heart failure, congenital defects, and vascular...
328
Imaging Studies for Cardiovascular System II:Types of Echocardiography01:20

Imaging Studies for Cardiovascular System II:Types of Echocardiography

432
Echocardiography plays a role in assessing cardiac health and detecting heart conditions, with various types providing critical insights for diagnosis and treatment.
Types of Echocardiography
Transthoracic Echocardiography (TTE)
TTE is the most common type of echocardiogram which involves placing a transducer on the patient's chest, emitting sound waves to create heart images. TTE is invaluable for evaluating the heart's size, structure, and motion, making it particularly useful for...
432
Imaging Studies for Cardiovascular System I:Echocardiography01:17

Imaging Studies for Cardiovascular System I:Echocardiography

533
Cardiac imaging studies encompass a wide range of noninvasive and minimally invasive techniques designed to visualize the heart's structure and function in detail. One such technique is echocardiography, which uses high-frequency ultrasound waves to produce detailed images of the heart, known as echocardiograms.
Indications: Echocardiography is utilized to diagnose heart failure, valve disorders, and myocardial infarction. It also assesses cardiac structures' size, shape, and motion,...
533
Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT01:25

Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT

169
Calcium-Scoring CT ScanA calcium-scoring CT scan, also known as coronary artery calcium (CAC) scan, detects calcium deposits in the coronary arteries. This test assesses the risk of coronary artery disease (CAD), which can lead to cardiovascular events such as angina, heart failure, and sudden cardiac arrest.A calcium-scoring CT scan is generally recommended for individuals at intermediate risk of CAD without symptoms. It includes:Men aged 40-75 and women aged 50-75: Especially those with a...
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Related Experiment Video

Updated: Oct 25, 2025

Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
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Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging

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Optical clearing in cardiac imaging: A comparative study.

Camilla Olianti1, Francesco Giardini1, Erica Lazzeri1

  • 1European Laboratory for Non-Linear Spectroscopy, Sesto Fiorentino, 50019, Italy.

Progress in Biophysics and Molecular Biology
|August 6, 2021
PubMed
Summary
This summary is machine-generated.

Optical clearing of mouse hearts using uDISCO, CLARITY, and SHIELD methods revealed varying tissue preservation and transparency. CLARITY offers deep imaging despite drawbacks, making it suitable for human cardiac tissue analysis.

Keywords:
Cardiovascular researchFluorescence microscopyLight-sheet microscopyTissue clearing

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Mesoscopic Optical Imaging of Whole Mouse Heart
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Area of Science:

  • Biomedical Engineering
  • Tissue Clearing
  • Optical Imaging

Background:

  • Achieving successful 3D reconstructions of entire hearts via optical clearing remains challenging.
  • Existing clearing protocols, often optimized for brain tissue, require significant adaptation for cardiac applications.
  • Cardiac tissue's unique structure necessitates specialized approaches for effective optical clearing.

Purpose of the Study:

  • To evaluate and optimize three distinct optical clearing methodologies (uDISCO, CLARITY, SHIELD) for mouse cardiac tissue.
  • To compare the efficacy of each method regarding tissue preservation, transparency, and staining.
  • To assess the suitability of optimized protocols for human cardiac tissue, including diseased hearts.

Main Methods:

  • Application and optimization of uDISCO, CLARITY, and SHIELD protocols on mouse cardiac tissue.
  • Comparative analysis of tissue features: preservation, transparency, and staining.
  • Adaptation and application of the CLARITY protocol to human cardiac tissue (control and hypertrophic cardiomyopathy).

Main Results:

  • uDISCO resulted in significant fiber delamination, compromising structural analysis reliability.
  • CLARITY provided high transparency and deep dye penetration but led to reduced tissue robustness and long processing times.
  • SHIELD showed promise for tissue maintenance and transparency, though homogeneous staining requires further development.

Conclusions:

  • CLARITY, despite its limitations, is the most effective method for deep imaging of labeled cardiac samples.
  • Optimized CLARITY protocol is applicable to human cardiac tissue, enabling imaging of both healthy and diseased hearts.
  • Further research is needed to refine SHIELD for consistent staining across entire cardiac specimens.