Variable metal resistance of P. putida CZ1 biofilms in different environments suggests its remediation application...

Pseudomonas putida is potentially used in the bioremediation of heavy metals (HMs). Its response to different HMs in different environments is still not fully understood. This study investigated resistance against 12 kinds of metals by P. putida CZ1 planktonic cells and its biofilm in LB and mineral medium (MM). P. putida CZ1 biofilms have high resistance and accumulation capacity for Cu²⁺, Zn²⁺, Pb²⁺, Fe³⁺, Mn²⁺, Al³⁺ and Ni²⁺, but less resistance to Co²⁺, Cd²⁺, Cr₂O₇^2-, Ag⁺ and Hg²⁺. Biofilms were 2-8 times more resistant to Cu²⁺ and Zn²⁺ than planktonic cells. There was a strong correlation between the P content and the accumulation of Cu²⁺, Zn²⁺, Fe³⁺, Mn²⁺, Pb²⁺, Ni²⁺and Al³⁺ respectively. Confocal laser scanning microscopy (CLSM) combined with live/dead staining study found that cells in the biofilms can keep viable after 36 h under MIC of Cu²⁺ or Zn²⁺ both in LB and MM. When the metal concentration increased, cells can be killed gradually. For Cu²⁺, Zn²⁺, Fe³⁺, Mn²⁺, Pb²⁺ and Ni²⁺, higher resistance was found in MM (2-4 times higher) than in LB and higher accumulation of these metals were also found in MM. P. putida CZ1 biofilm cultured in MM with citric acid as carbon source had stronger resistance and accumulation ability to Cu²⁺, Zn²⁺, Pb²⁺, Fe³⁺, Mn²⁺, and Ni²⁺. This suggested that P. putida CZ1 had greater remediation potential for these metals in organic acid rich environments.