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Related Experiment Videos

Free diffusion coefficient of ionic calcium in cytoplasm.

B S Donahue1, R F Abercrombie

  • 1Department of Physiology, Emory University School of Medicine, Atlanta, Georgia 30322.

Cell Calcium
|December 1, 1987
PubMed
Summary

The free diffusion coefficient of calcium ions (Ca) in Myxicola axoplasm is 5.3 x 10(-6) cm2 s-1. This measurement was achieved under conditions minimizing calcium sequestration and chelation.

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Area of Science:

  • Cellular Physiology
  • Ion Transport Mechanisms

Background:

  • Calcium ions (Ca) play crucial roles in cellular processes.
  • Understanding calcium diffusion is vital for cellular function.
  • Myxicola axoplasm serves as a model system for studying axonal transport.

Purpose of the Study:

  • To determine the free diffusion coefficient of ionic calcium (Ca) in isolated Myxicola axoplasm.
  • To investigate the influence of calcium buffering and sequestration on Ca diffusion.
  • To explore calcium ion migration dynamics in untreated axoplasm.

Main Methods:

  • Utilized radioactive 45Ca to track calcium ion migration.
  • Employed inhibitors, energy deprivation, and chelation site saturation to control for calcium binding.
  • Measured diffusion coefficients under varying free calcium concentrations and diffusion times.

Main Results:

  • A diffusion coefficient of 5.3 x 10(-6) cm2 s-1 was measured for Ca in isolated Myxicola axoplasm when sequestration and chelation were minimized.
  • The diffusion coefficient remained consistent across a range of free calcium concentrations (10-100 microM) and diffusion times (10-20 minutes).
  • Calcium migration in untreated axoplasm exhibited complex behavior, not fitting a single diffusion coefficient, suggesting potential bulk movement of calcium buffers.

Conclusions:

  • The intrinsic diffusion coefficient of free Ca in Myxicola axoplasm is approximately 5.3 x 10(-6) cm2 s-1.
  • Calcium buffering and sequestration significantly impact observed calcium ion mobility in the axoplasm.
  • The complex Ca migration in untreated axoplasm indicates the involvement of Ca-binding proteins and potential bulk flow.

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