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Dysregulation of PI3K/Akt/PTEN Pathway in Canine Mammary Tumor

Soo-Hyeon Kim1, Byung-Joon Seung1, Seung-Hee Cho1

  • 1Department of Veterinary Pathology, College of Veterinary Medicine, Konkuk University, Seoul 05025, Korea.

Animals : an Open Access Journal From MDPI
|August 7, 2021
PubMed

Insights

The PI3K/Akt/PTEN pathway is altered in canine mammary tumors. While PIK3CA H1047R mutations are present, only PTEN expression changes correlate with this specific mutation in canine mammary cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Veterinary Medicine

Background:

  • The PI3K/Akt/PTEN signaling pathway is crucial in tumorigenesis.
  • PIK3CA mutations, particularly H1047R, are common in human and canine cancers.
  • The characteristics of PIK3CA-mutated canine mammary tumors remain unclear.

Purpose of the Study:

  • To investigate the PI3K/Akt/PTEN pathway alterations in canine mammary tumors.
  • To determine the correlation between PIK3CA H1047R mutation and pathway component dysregulation.

Main Methods:

  • Analysis of 83 canine mammary samples (normal, adenoma, low-grade carcinoma, high-grade carcinoma).
  • Genomic DNA extraction, PCR amplification, and Sanger sequencing for PIK3CA mutation analysis.
  • Immunohistochemistry for PIK3CA, Akt, p-Akt, PTEN expression and RNA in situ hybridization for Akt2.

Main Results:

  • PIK3CA H1047R mutation found in 14.3% of tumor samples.
  • Dysregulation of p-Akt, Akt2, and PTEN observed in mammary tumors.
  • Only PTEN dysregulation was significantly associated with the PIK3CA H1047R mutation.

Conclusions:

  • Dysregulation of the PI3K/Akt/PTEN pathway is a feature of canine mammary tumors.
  • This pathway dysregulation is not directly linked to PIK3CA H1047R mutation, except for PTEN expression.
Keywords:
PI3K/Akt/PTEN pathwayPIK3CA H1047R mutationdogsmammary neoplasms

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