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Related Experiment Video

Updated: Oct 25, 2025

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Published on: December 28, 2015

Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes.

Laura Abaandou^1,2, David Quan¹, Joseph Shiloach¹

¹Biotechnology Core Laboratory National Institutes of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, MD 20892, USA.

|August 7, 2021

Summary

Human HEK293 cells are crucial for biotherapeutics. Genetic and bioprocess engineering significantly enhances their growth and production, improving performance for human biologics.

Keywords:

HEK293 cell line engineering recombinant protein production

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Published on: December 28, 2015

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Published on: May 7, 2018

Area of Science:

Biotechnology
Cell Line Engineering
Biopharmaceutical Production

Background:

HEK293 cells are a key human cell line for biotherapeutic production due to their origin and transfection ease.
Current HEK293 cell performance is suboptimal compared to non-human cell lines like CHO and NSO.
Significant improvements are needed to enhance HEK293 cell growth and productivity.

Purpose of the Study:

To review advancements in HEK293 cell line engineering for biopharmaceutical production.
To summarize global engineering strategies and functional genomic tools used for HEK293 improvement.
To highlight achievements in optimizing HEK293 cells for enhanced biomanufacturing.

Main Methods:

Gene modification targeting cellular processes (proliferation, apoptosis, metabolism, etc.).
Bioprocess, media, and vector optimization strategies.
Application of functional genomic tools for gene identification and targeted engineering.

Main Results:

Genetic engineering has substantially improved HEK293 cell growth and production.
Optimization of cellular processes enhances biotherapeutic output.
Functional genomics aids in identifying key genes for targeted cell line improvement.

Conclusions:

HEK293 cell line engineering has significantly advanced biotherapeutic production capabilities.
Targeted genetic modifications and bioprocess optimization are key to enhancing HEK293 performance.
Continued research in cell line engineering will further improve the suitability of HEK293 for biologics manufacturing.