Development of a reference method for determining urinary oxalate by means of isotope dilution-mass spectrometry (ID-MS) and its usefulness in testing existing assays for urinary oxalate
Related Experiment Videos
W Koolstra1, B G Wolthers, M Hayer
1Central Laboratory for Clinical Chemistry, University Hospital, Groningen, The Netherlands.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|December 1, 1987
Abstract:
An accurate and reproducible GC-MS reference method for determining urinary oxalate is presented. With forty 24-h urines, comparisons were made between this reference method and four other existing procedures: a GC method, an HPLC method and two enzymatic assays. The results of the first two methods were in accordance with the GC-MS method. The performance of the enzymatic kits was less satisfactory. The use of a GC-MS reference method in evaluating existing and newly developed methods is recommended.