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High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
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Complexome Profiling: Assembly and Remodeling of Protein Complexes.

Ilka Wittig1, Pedro Felipe Malacarne1

  • 1Institute for Cardiovascular Physiology, Goethe University, 60590 Frankfurt, Germany.

International Journal of Molecular Sciences
|August 7, 2021
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Complexome profiling uses mass spectrometry to identify protein assemblies and their dynamics in cells. This technique reveals how protein complexes form, change, and function in health and disease.

Keywords:
assemblycomplexome profilingdata repositoriesmass spectrometryprotein complexesprotein–protein interactionremodeling

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Proteins function within complexes with other biomolecules like nucleic acids, carbohydrates, and lipids.
  • Protein complex formation can be transient, stable, or dynamic, regulated by cellular conditions.

Purpose of the Study:

  • To review the applications of complexome profiling for studying protein assemblies.
  • To summarize the use of complexome profiling in understanding protein complex stability and remodeling in health and disease.

Main Methods:

  • Complexome profiling is a mass spectrometry-based technique.
  • It employs mild separation methods including native gel electrophoresis and density gradient centrifugation.
  • Quantitative mass spectrometry is used to generate inventories of protein assemblies.

Main Results:

  • Complexome profiling can identify protein assemblies from single subunits to large macromolecular complexes.
  • The method provides insights into the stability and dynamics of protein complexes.
  • Applications span various biological contexts, including health and disease states.

Conclusions:

  • Complexome profiling is a powerful tool for mapping cellular protein assemblies.
  • It offers a comprehensive view of protein complex dynamics and their roles in biological processes.
  • This technique aids in understanding disease mechanisms at the molecular level.