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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
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Cholesterol Sequestration from Caveolae/Lipid Rafts Enhances Cationic Liposome-Mediated Nucleic Acid Delivery into

Santhosh Chandar Maddila1,2, Chandrashekhar Voshavar3, Porkizhi Arjunan1

  • 1Centre for Stem Cell Research (CSCR) (A Unit of inStem, Bengaluru), Christian Medical College Campus, Bagayam, Vellore 632002, TN, India.

Molecules (Basel, Switzerland)
|August 7, 2021
PubMed
Summary

Modulating endothelial cell endocytosis with cholesterol-sequestrating agents significantly enhances nucleic acid delivery. Nystatin treatment boosted transfection efficacy by 2-3 fold in liver sinusoidal endothelial cells.

Keywords:
cationic lipidscaveolae-mediated endocytosisclathrin-mediated endocytosisendothelial cellsmacropinocytosistransfection

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