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Ion Chromatography with Pulsed Amperometric Detection for Determining Cyanide in Urine and Meconium Samples

Ewa Jaszczak-Wilke1, Krystyna Kozioł1, Bogumiła Kiełbratowska2

  • 1Department of Analytical Chemistry, Faculty of Chemistry, Gdansk University of Technology, 11/12 Narutowicza Str., 80-233 Gdansk, Poland.

Molecules (Basel, Switzerland)
|August 7, 2021
PubMed

Insights

This study measured cyanide levels in newborn meconium and maternal urine, revealing significant exposure. Ion chromatography with pulsed amperometric detection (IC-PAD) effectively quantified these trace cyanide concentrations.

Area of Science:

  • Environmental Toxicology
  • Analytical Chemistry
  • Neonatal Health

Background:

  • Parental lifestyle choices, including addictions and diet, impact child development.
  • Newborn meconium is a valuable source for assessing prenatal exposure to xenobiotics.
  • Maternal cyanide exposure can affect fetal development.

Purpose of the Study:

  • To determine cyanide concentrations in meconium and maternal urine.
  • To validate ion chromatography with pulsed amperometric detection (IC-PAD) for cyanide analysis in human biological matrices.
  • To establish IC-PAD as a novel method for meconium cyanide determination.

Main Methods:

  • Analysis of cyanide in urine and meconium samples using ion chromatography with pulsed amperometric detection (IC-PAD).
  • Optimization of chromatographic conditions for accurate and sensitive cyanide detection.
  • Validation of the IC-PAD method for repeatability and linearity.

Main Results:

  • Cyanide concentrations in maternal urine ranged from 1.30-25.3 μg L-1.
  • Cyanide concentrations in meconium samples ranged from 1.54 μg L-1 to 24.9 μg L-1.
  • The IC-PAD system demonstrated excellent repeatability (R < 3%) and linearity (1-100 μg L-1).

Conclusions:

  • IC-PAD is an effective tool for monitoring trace cyanide levels in human body fluids, including meconium.
  • This study represents the first application of IC-PAD for cyanide ion determination in meconium.
  • Findings highlight the potential for prenatal cyanide exposure assessment.

Keywords:
PLEcyanideion chromatographymeconiumtobacco smokingurine

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