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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Related Experiment Video

Updated: Oct 25, 2025

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Published on: May 25, 2018

N-Terminomics Strategies for Protease Substrates Profiling.

Mubashir Mintoo¹, Amritangshu Chakravarty¹, Ronak Tilvawala¹

¹Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA.

Molecules (Basel, Switzerland)

|August 7, 2021

Summary

N-terminomics, a mass spectrometry technique, identifies protease substrates by analyzing protein fragments. This method aids in understanding protease roles in diseases and advancing protease research.

Keywords:

CHOPS COFRADIC N-terminomics TAILS protease substrates subtiligase

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Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Proteases are crucial enzymes regulating biological events through proteolysis.
Dysregulated protease activity is linked to various diseases, including cancer and infections.
Understanding protease function requires identifying their substrates and cleavage sites.

Purpose of the Study:

To review N-terminomics techniques for identifying protease substrates.
To highlight the strengths, weaknesses, and limitations of N-terminomics.
To provide examples of N-terminomics applications in physiological and disease contexts.

Main Methods:

N-terminomics utilizes mass spectrometry to analyze labeled N-terminal peptides.
Enrichment of native and neo-N-termini peptides generated by proteolysis.
Direct profiling of protease substrates from complex biological samples.

Main Results:

N-terminomics enables direct identification of protease substrates in vivo.
The technique provides insights into the cellular proteolytic landscape.
Successful applications demonstrate its utility in various biological systems.

Conclusions:

N-terminomics is a powerful tool for protease substrate identification.
This technique advances the understanding of protease roles in health and disease.
Future developments hold promise for further insights into protease biology.