[Effect of Vitamin D3 to Platelet Activation Mediated by Tumor Cell Culture Medium]

To investigate the effect of vitamin D3 to platelet activation by tumor cell culture medium.

The peripheral blood platelets of BALB/c mice were isolated. The platelets were activated in 4T1 culture fluid for 24 h. The platelets were divided into 7 groups: control group, activation group, 1 nmol/L vitamin D3 group, 10 nmol/L vitamin D3 group, 50 nmol/L vitamin D3 group, 100 nmol/L vitamin D3 group, and positive drug (0.1 μmol/L eptifibatide) group. CCK-8 assay was used to detect the platelet proliferation at 24, 48 and 72 h. Flow cytometry was used to detect the expression of CD61 and CD62p and receptor for advanced glycation end products (RAGE) at 24, 48 and 72 h. ELISA was used to detect the level of platelet-endothelial cell adhesion molecule-1 (PECAM-1) at 24, 48 and 72 h.

The CD41⁺/CD61⁺ and CD41⁺/CD62p⁺ ratio, PECAM-1 content and RAGE expression of platelets in activated group were all significantly increased as compared with those in control group (P<0.05). Compared with the activated group, the platelet proliferation, proportion of CD41⁺/CD61⁺ and CD41⁺/CD62p⁺, content of PECAM-1 and RAGE expression in vitamin D3 groups were all decreased (P<0.05).

Vitamin D3 shows antiplatelet effect and can inhibit platelet proliferation and activation.