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[Clinical Application of Screening Cell Combination Method in the Prediction of Red Blood Cell Alloantibody]
Xiao-Fei Li1, Rong Lang2, Xue-Qin Diao3
1Department of Blood Transfusion, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China ,E-mail:lixiaofei@ccmu.edu.cn.
Insights
The combined method effectively identifies red blood cell alloantibodies in the Chinese population, improving diagnostic accuracy. This approach optimizes reagent use for screening irregular antibodies.
Area of Science:
- Transfusion Medicine
- Immunology
- Clinical Diagnostics
Background:
- Red blood cell alloantibodies can cause transfusion reactions.
- Accurate identification of alloantibodies is crucial for safe blood transfusions.
- Existing screening methods may have limitations in sensitivity and specificity.
Purpose of the Study:
- To evaluate the clinical utility of a combined screening cell method for predicting red blood cell alloantibodies.
- To establish a basis for improved clinical diagnosis of alloimmunization.
Main Methods:
- Screening of 9,680 samples using an automated blood group instrument.
- Identification of 79 positive alloantibodies using a combined method with four different screening cell sets.
- Comparative analysis with the Panocell-16 cell panel.
- Validation using external quality assessment samples from China and WHO.
Main Results:
- The combined method identified a wide range of alloantibodies, including anti-Mia and anti-Leª.
- Panocell-16 missed 7 cases of anti-Mia and 1 case of anti-Leª.
- The combined method achieved 100% accuracy in identifying antibodies from external quality assessment samples.
- No significant difference was observed between the combined method and Panocell-16 in overall identification.
Conclusions:
- The combined screening cell method is effective for identifying red blood cell alloantibodies in the Chinese population.
- This method allows for efficient screening of irregular antibodies while conserving reagents.
- The findings support the clinical application of the combined method for enhanced diagnostic accuracy.
Objective:
To explore the clinical application of screening cell combination method in the prediction of red blood cell alloantibody, so as to provide basis for clinical diagnosis.
Methods:
From October 2018 to April 2020, 9 680 samples were screened with automatic blood group instrument, 79 patients with positive alloantibodies were identified by 4 sets of screening cells from different manufacturers (referred to as combined method). At the same time, cell panel Panocell-16 was used for comparative analysis. Meanwhile, the combined method was also used to identify the antibodies of 20 samples from National Center for Clinical Laboratories external quality assessment (EQA) in China and 12 samples from WHO EQA.
Results:
The 79 alloantibodies included anti-Mia antibody (7 cases), anti-M antibody (13 cases), anti-Lea antibody (9 cases), anti-P1 antibody (2 cases), anti-E antibody (22 cases), anti-c + E antibody (9 cases), anti-D antibody (4 cases), anti-e antibody (2 cases), anti-C + e antibody (3 cases), anti-C antibody (3 cases), anti-H antibody (1 case), anti-Fyb antibody (1 case), anti-E + M antibody (1 case), autoantibody + anti-E (2 cases). However, 7 cases of anti-Mia antibody and 1 case of anti- Lea antibody were missed in Panocell-16 identification results. The results of antibody identification in 20 samples from National Center for Clinical Laboratories EQA in China and 12 samples from WHO EQA were 100% accurate by combination method. The identification results between combined method identification and Panocell-16 identification showed no significant difference.
Conclusion:
The combined method can identify the alloantibodies of red blood cells in Chinese population. The screening cells can be used for screening of irregular antibodies without wasting reagents at the same time.
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