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In vitro Cell Culture Model for Toxic Inhaled Chemical Testing
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[Evaluation of the damaging and transforming effect of sodium nitrite on cultured lung cells]

N D Osin'kovskaia

    Eksperimental'Naia Onkologiia
    |January 1, 1987
    PubMed

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    2° Amines to N-Nitrosamines: Reaction with NaNO201:20

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    Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.