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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Synthetic Biology

Background:

  • Biological systems utilize diverse agents like peptides and toxins to disrupt lipid membranes.
  • These membrane-disrupting agents have applications in biosensing and therapeutics.
  • Synthetic approaches can create novel agents for membrane manipulation.

Purpose of the Study:

  • To introduce a novel class of synthetic, DNA-based particles for lipid membrane disruption.
  • To demonstrate programmable control over particle-induced membrane permeabilization and vesicle collapse.
  • To explore the potential of these DNA particles in pathogen trapping.

Main Methods:

  • Self-assembly of DNA-based particles with a membrane-adhesive cholesterol-DNA core and a protective DNA corona.
  • Selective displacement of the corona using molecular cues to expose the core.
  • Interaction studies with synthetic lipid vesicles to assess membrane disruption and collapse.
  • Investigation of particle aggregation and gel formation.
  • Demonstration of bacterial trapping using DNA aggregates.

Main Results:

  • Programmable DNA particles were successfully synthesized with tunable size.
  • Exposure of the cholesterol-DNA core led to adhesion to lipid vesicles.
  • Adhesion induced membrane permeabilization and subsequent vesicle collapse.
  • Particle coalescence formed DNA-based gels that enveloped vesicles.
  • DNA aggregates effectively trapped E. coli bacteria, mimicking natural immune processes.

Conclusions:

  • Synthetic DNA particles offer a controllable platform for lipid membrane disruption.
  • The triggered release mechanism allows for targeted membrane interaction.
  • The formation of DNA gels presents a novel strategy for pathogen immobilization.
  • This work bridges synthetic biology and materials science for potential therapeutic and diagnostic applications.