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Author Correction: A simple and robust real-time qPCR method for the detection of PIK3CA mutations

Virginia Alvarez-Garcia1, Clare Bartos2,3, Ieva Keraite4,5

  • 1Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS, UK. v.alvarez_garcia@hw.ac.uk.

Scientific Reports
|August 7, 2021
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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