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Updated: Sep 1, 2026

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
Published on: September 8, 2011
Interactions between gallamine and muscarinic receptors: allosterism and subpopulation specificity are separate
1Department of Psychiatry, University of Vermont College of Medicine, Burlington 05405.
Abstract:
The quaternary antagonist gallamine discriminates subpopulations of muscarinic receptors in the forebrain and brainstem of the rat and also modulates the binding of other muscarinic ligands via an allosteric mechanism in both brain regions. The calcium channel antagonist verapamil exhibits a similar allosteric effect in both regions (at 100 microM) but does not differentiate the subpopulations that are defined by gallamine. On the other hand, N-methylatropine does not produce the allosteric effect at 100 microM, but shows a high degree of selectivity (about 1,000-fold) for the gallamine-defined subpopulations. These studies indicate that the allosterism and subpopulation specificity of gallamine are separate phenomena and may be examined in isolation by appropriate ligands.
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