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Thiophosphorylation of skeletal muscle sarcoplasmic reticulum in porcine malignant hyperpyrexia
A T Sim1, M D White, M A Denborough
1Department of Medicine and Clinical Science, John Curtin School of Medical Research, Australian National University, Canberra.
The International Journal of Biochemistry
|January 1, 1987
Abstract:
1. The basal, Ca2+-dependent and Mg2+-dependent thiophosphorylation of malignant hyperpyrexia-susceptible (MHS) porcine skeletal muscle was investigated. 2. Seven major proteins of Mr 100,000-11,000 were substrates for thiophosphorylation. 3. Sodium molybdate significantly elevated all levels of thiophosphorylation in control sarcoplasmic reticulum, but did not effect the Ca2+-dependent thiophosphorylation of MHS samples. 4. These results suggest that MHS sarcoplasmic reticulum may have altered sensitivity to protein phosphatase inhibition.