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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
Published on: January 9, 2020
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Spectral Markers for T Cell Death and Apoptosis-A Pilot Study on Cell Therapy Drug Product Characterization Using
Lifu Xiao1, Sagar Shelake1, Maria Ozerova1
1Large Molecule Drug Product Development, Biotherapeutics Development, Janssen Research & Development LLC, Spring House, PA 19477, USA.
Journal of Pharmaceutical Sciences
|August 8, 2021
Summary
Raman spectroscopy can identify T cell changes during cryopreservation, correlating with apoptosis markers. This label-free technique supports cell therapy drug development and process control.
Area of Science:
- Biotechnology
- Immunology
- Analytical Chemistry
Background:
- Cell therapy drug products rely on T cell quality.
- Cryopreservation can induce T cell apoptosis, impacting efficacy.
- Current T cell characterization methods may be complex or require labels.
Purpose of the Study:
- To evaluate Raman spectroscopy as a tool for T cell characterization.
- To correlate Raman signals with T cell apoptosis markers.
- To assess the utility of Raman spectroscopy in cell therapy development.
Main Methods:
- Raman spectroscopy was applied to T cells.
- Flow cytometry was used to detect apoptosis markers.
- Statistical analysis correlated Raman signals with flow cytometry data.
Main Results:
- Statistically significant correlations were found between Raman signals and T cell apoptosis markers.
- Raman spectroscopy provided label-free measurements of T cell characteristics.
- The technique demonstrated sensitivity to changes during cryopreservation.
Conclusions:
- Raman spectroscopy is a viable tool for T cell characterization in cell therapy.
- It offers label-free insights into T cell apoptosis during cryopreservation.
- This method can aid in cell therapy product design and process control.
