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Updated: Oct 25, 2025

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
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Spectral Markers for T Cell Death and Apoptosis-A Pilot Study on Cell Therapy Drug Product Characterization Using

Lifu Xiao1, Sagar Shelake1, Maria Ozerova1

  • 1Large Molecule Drug Product Development, Biotherapeutics Development, Janssen Research & Development LLC, Spring House, PA 19477, USA.

Journal of Pharmaceutical Sciences
|August 8, 2021
PubMed

Keywords:
Apoptosis markersCell therapyCryopreservationDrug product characterizationFlow cytometryRaman spectroscopy

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Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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