Related Experiment Video
Updated: Oct 25, 2025
Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus Subtypes
Published on: July 26, 2019
[Serological and molecular studies of a rare A subgroup]
Objective:
To determine the genotype of an individual suspected for Aw through DNA sequencing.
Methods:
Serologic testing was carried out with standard methods. Exons 6 and 7 of the ABO genes were amplified by PCR and subjected to direct sequencing or sequenced after gene cloning.
Results:
Serological testing showed that the forward typing and reverse typing were Aw and A, respectively. DNA sequencing revealed that the individual has carried an Aw allele and an O allele. Haplotype sequencing of each allele has revealed a nt543 variant (543G>C) in the Aw allele.
Conclusion:
The individual was verified as a rare A subtype, which was previously unreported in mainland China.
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