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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Overview of Electron Microscopy01:25

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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
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Related Experiment Video

Updated: Jul 5, 2026

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy
06:49

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Published on: May 31, 2017

A method for locating individual leukocytes for comparative study by light and scanning electron microscopy.

S A Pier, J E Gallagher

    American Journal of Veterinary Research
    |January 1, 1978
    PubMed
    Summary

    This study introduces a novel method for identifying specific leukocytes and platelets in stained blood films. Researchers can now compare cell structures using both light and scanning electron microscopy for detailed analysis.

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    Area of Science:

    • Hematology
    • Microscopy
    • Cell Biology

    Background:

    • Comparative analysis of blood cells requires advanced imaging techniques.
    • Light microscopy and scanning electron microscopy offer complementary views of cell morphology.

    Purpose of the Study:

    • To develop a method for correlating light microscopy images of stained blood cells with their scanning electron microscopy surface morphology.
    • To enable detailed comparative studies of individual leukocytes and platelets.

    Main Methods:

    • Cells in Wright's stained blood films were photographed and their fields marked.
    • Slides were broken, and marked sections were prepared for scanning electron microscopy (SEM).
    • Cells were located in SEM via the marked fields for 3D surface imaging.

    Main Results:

    • The method successfully located marked cells in SEM.
    • Three-dimensional surface morphology from SEM was compared with 2D light microscopy images.
    • Distinct characteristics of individual leukocyte and platelet types were observed.

    Conclusions:

    • This technique allows for direct comparison of light and SEM imaging of the same individual blood cells.
    • It provides a valuable tool for detailed hematologic and cytologic investigations.