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Total sequential solid phase synthesis of rabbit skeletal troponin C calcium binding site III
1Faculty of Pharmacy, University of Manitoba, Winnipeg, Canada.
International Journal of Peptide and Protein Research
|November 1, 1987
Abstract:
The total sequential solid phase synthesis of AcA98STnC(90-123)amide is described. The yield is comparable to that obtained previously using fragment condensation on the solid support. HPLC purification results in a product which displays greater calcium induced conformational changes than the peptide prepared by the fragment condensation method. However, the former has a dissociation constant for calcium (28 microM) seven times higher than the latter (4 microM).