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Harmonic optical tomography of nonlinear structures.

Chenfei Hu1,2,3, Jeffrey J Field4,5,6,3, Varun Kelkar1

  • 1Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Nature Photonics
|August 9, 2021
PubMed
Summary
This summary is machine-generated.

Harmonic optical tomography (HOT) offers a new way to image nonlinear materials by measuring complex harmonic fields. This technique overcomes limitations of conventional methods, enabling detailed 3D reconstruction of microscopic structures.

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Area of Science:

  • Optics
  • Microscopy
  • Biomedical Imaging

Background:

  • Second-harmonic generation (SHG) microscopy is a label-free imaging technique for non-centrosymmetric structures.
  • Conventional SHG microscopy faces challenges in solving the inverse scattering problem for nonlinear materials due to reliance on intensity signals from focused beams.

Purpose of the Study:

  • Introduce harmonic optical tomography (HOT) as a novel modality for imaging microscopic, nonlinear, and inhomogeneous objects.
  • Overcome limitations of conventional SHG microscopy for 3D reconstruction of nonlinear material properties.

Main Methods:

  • HOT utilizes interferometric measurement of the complex harmonic field.
  • Employs a scattering inverse model to reconstruct the 3D distribution of harmonophores.
  • Achieves axial sectioning through momentum conservation of broadband fields.

Main Results:

  • Demonstrated HOT operation with experiments on a beta-barium borate crystal.
  • Successfully reconstructed various biological specimens using HOT.
  • Validated the applicability of the HOT approach to any coherent nonlinear process.

Conclusions:

  • HOT provides a powerful new modality for label-free 3D imaging of nonlinear optical properties.
  • The technique enables precise reconstruction of harmonophore distributions in microscopic and inhomogeneous samples.
  • HOT's principles extend beyond second-order nonlinear materials to broader coherent nonlinear optical processes.