Related Experiment Video

Updated: Oct 25, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.1K

N6-Methyladenosine Modification Opens a New Chapter in Circular RNA Biology

Jun Wu1,2, Xin Guo3, Yi Wen1

  • 1Department of General Surgery and Pancreatic Injury and Repair Key Laboratory of Sichuan Province, The General Hospital of Western Theater Command, Chengdu, China.

Frontiers in Cell and Developmental Biology
|August 9, 2021
PubMed

Insights

N6-methyladenosine (m6A) modification regulates circular RNAs (circRNAs) metabolism, impacting immunity and diseases. This review explores m6A

Area of Science:

  • RNA epigenetics
  • Molecular biology
  • Gene regulation

Background:

  • N6-methyladenosine (m6A) is a prevalent mRNA modification in eukaryotes, influencing physiological processes and diseases.
  • Circular RNAs (circRNAs) are crucial in disease pathogenesis and exhibit widespread m6A modification.
  • m6A modification on circRNAs regulates their metabolism, including biogenesis, translation, degradation, and localization.

Purpose of the Study:

  • To review the roles of m6A in circRNA metabolism.
  • To summarize regulatory mechanisms of m6A-modified circRNAs in immunity and diseases.
  • To discuss challenges and future directions in studying m6A modification in circRNAs.

Main Methods:

  • Literature review of studies on m6A modification in circRNAs.
  • Analysis of regulatory mechanisms and biological functions.
  • Synthesis of current knowledge and identification of research gaps.

Main Results:

  • m6A modification is widespread in circRNAs and crucial for their metabolism.
  • m6A-modified circRNAs play significant roles in immune responses and various diseases.
  • This modification adds a new layer of regulatory control in biological processes.

Conclusions:

  • m6A modification of circRNAs is a key factor in RNA epigenetics.
  • Understanding m6A-circRNA interactions offers insights into disease mechanisms.
  • Further research is needed to fully elucidate the complexities and therapeutic potential.
Keywords:
N6-methyladenosinecircular RNAsdiseasesimmunitymetabolism

More Related Videos

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.6K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.9K

Related Concept Videos

RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.3K
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
34.3K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
11.6K
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
54.4K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.0K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
12.4K