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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Oct 25, 2025

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

A microfluidic cytometer for white blood cell analysis.

Tao Peng¹, Xinyue Su¹, Xingzhi Cheng²

¹Department of Biomedical Engineering, School of Life Science, Beijing Institute of Technology, Beijing, China.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|August 9, 2021

Summary

This summary is machine-generated.

This study introduces a microfluidic cytometer for improved white blood cell classification. The portable device uses hydrodynamic focusing and on-chip optics for accurate point-of-care blood cell analysis.

Keywords:

3D hydrodynamic focusing airborne microlens microfluidic cytometer white blood cells classification

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Area of Science:

Biomedical Engineering
Microfluidics
Cellular Analysis

Background:

Traditional cytometers face limitations in point-of-care testing for white blood cell classification.
Microfluidic technology offers potential for developing portable diagnostic devices.

Purpose of the Study:

To present a prototype microfluidic cytometer for counting and classifying white blood cells.
To demonstrate the integration of 3D hydrodynamic focusing and on-chip optics for enhanced cell analysis.

Main Methods:

Development of a microfluidic chip with a three-dimensional hydrodynamic focusing system.
Integration of an on-chip optical system with optical fibers and microlenses for cell excitation and detection.
Utilizing sheath and sample flow adjustments for precise horizontal and vertical cell focusing within the microchannel.

Main Results:

The microfluidic cytometer successfully counted and classified white blood cells.
The integrated system demonstrated effective single-cell excitation and detection.
Validation was performed using clinical blood samples, showing promising performance.

Conclusions:

The developed microfluidic cytometer prototype shows potential for improving white blood cell classification at the point-of-care.
This technology could lead to more accessible and efficient blood cell diagnostic tools.
Further development may enhance the performance and applicability of microfluidic cytometry.