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Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
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Understanding the Interactions Between the Ocular Surface Microbiome and the Tear Proteome
Denise C Zysset-Burri1,2, Irina Schlegel1, Joel-Benjamin Lincke1
1Department of Ophthalmology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Investigative Ophthalmology & Visual Science
|August 9, 2021
Summary
The human ocular surface microbiome and tear proteome interact, influencing immune defense. Understanding this relationship is key for developing new treatments for eye surface diseases.
Area of Science:
- Ophthalmology
- Microbiology
- Proteomics
Background:
- The ocular surface hosts a complex microbiome.
- The tear proteome plays a crucial role in ocular health and disease.
- Understanding the interplay between these two is vital for ocular disease pathogenesis.
Purpose of the Study:
- To investigate the relationship between the ocular surface microbiome and tear proteome in humans.
- To elucidate the role of this interplay in the pathogenesis of ocular surface diseases.
Main Methods:
- Whole-metagenome shotgun sequencing of ocular surface swabs (lid and conjunctiva).
- Tear proteome analysis using chromatography tandem mass spectrometry.
- Association studies correlating microbiome composition and function with proteome data.
Main Results:
- Identified 229 taxa, with Actinobacteria and Proteobacteria as dominant phyla.
- Detected significant differences in microbial composition between lid and conjunctival swabs.
- Found correlations between specific microbial taxa/functions and tear proteome components, including those related to metabolism, immune defense, and vision.
Conclusions:
- The ocular surface microbiome is significantly associated with the tear proteome.
- This association plays a role in human immune defense mechanisms.
- Findings may inform novel therapeutic strategies for ocular surface diseases.
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