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A kinetic method for quantification of aspartate aminotransferase isoenzymes
1Departament de Bioquímica i Fisiologia, Facultat de Química, Universitat de Barcelona, Spain.
Journal of Biochemical and Biophysical Methods
|November 1, 1987
Abstract:
A spectrophotometric assay is proposed to determine the levels of aspartate aminotransferase (AAT) isoenzymes from chicken liver by a steady-state kinetic method which depends on the differential inhibition of these isoenzyme forms by high concentrations of substrate 2-oxoglutarate at pH 6.2. The use of a standard curve permits the determination of the percentage of chicken liver c-AAT and m-AAT isoenzymes. This method yields results in good correlation with those achieved by different extent adipate inhibition and by differential centrifugation.