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[Vibrion variability under the action of antibiotics]
Antibiotiki
|February 1, 1978
Summary
Antibiotic resistance in Vibrio cholerae and NAG-vibrios was induced by streptomycin, tetracycline, and monomycin. Induced resistance altered bacterial properties but was unstable, reverting after antibiotic withdrawal.
Area of Science:
- Microbiology
- Bacteriology
- Antibiotic Resistance
Background:
- Vibrio cholerae and NAG-vibrios are significant bacterial pathogens.
- Antibiotic resistance is a growing global health concern.
- Understanding resistance mechanisms is crucial for effective treatment.
Purpose of the Study:
- To generate and characterize antibiotic-resistant variants of V. cholerae and NAG-vibrios.
- To investigate the stability of induced antibiotic resistance.
- To assess changes in diagnostic properties of resistant strains.
Main Methods:
- Repeated passages of V. cholerae and NAG-vibrios on media with increasing antibiotic concentrations (tetracycline, streptomycin, monomycin).
- Evaluation of resistance levels and phenotypic changes (colony morphology, fermentative properties, agglutinability).
- Assessment of property stability after removal of antibiotics.
Main Results:
- Streptomycin induced the highest number of resistant variants with the highest resistance levels (up to 8000 gamma/ml).
- V. cholerae variants showed altered colony morphology, fermentative properties, and reduced agglutinability.
- NAG-vibrio variants exhibited changes in colony structure but retained fermentative properties and agglutination capacity.
- Induced changes were unstable, disappearing after 2-3 passages on antibiotic-free media.
Conclusions:
- Antibiotic pressure can induce resistance and alter phenotypic characteristics in V. cholerae and NAG-vibrios.
- Induced resistance and associated phenotypic changes are transient.
- These findings have implications for bacterial diagnostics and surveillance.