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Non-coding Natural Antisense Transcripts: Analysis and Application.

Julian C Krappinger1, Lilli Bonstingl2, Katrin Pansy3

  • 1Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center for Cell Signaling, Metabolism and Aging, Medical University of Graz, Neue Stiftingtalstraße 6/II, 8010 Graz, Austria; Christian Doppler Laboratory for innovative Pichia pastoris host and vector systems, Division of Cell Biology, Histology and Embryology, Medical University of Graz, Neue Stiftingtalstraße 6/II, 8010 Graz, Austria.

Journal of Biotechnology
|August 9, 2021
PubMed
Summary
This summary is machine-generated.

Non-coding natural antisense transcripts (ncNATs) are regulatory RNAs involved in cancer. Analyzing these complex molecules using RNA-sequencing presents challenges but offers potential for cancer biomarkers and treatments.

Keywords:
biomarkersliquid biopsylong non-coding RNAsnatural antisense transcriptsstrand-specific RNA-seq

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Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • Non-coding natural antisense transcripts (ncNATs) are regulatory RNA molecules with roles in biological and pathological processes, including cancer.
  • The study of ncNATs is nascent, with limited annotations, expression data, and functional characterizations compared to protein-coding genes.
  • Significant gaps exist in the analysis and characterization of these regulatory transcripts.

Purpose of the Study:

  • To review non-coding natural antisense transcripts (ncNATs) from an analytical perspective.
  • To highlight the application of high-throughput sequencing strategies like RNA-sequencing for ncNAT investigation.
  • To discuss the challenges and potential of ncNATs as cancer biomarkers and therapeutic targets.

Main Methods:

  • Review of current literature on ncNAT analysis.
  • Discussion of high-throughput sequencing strategies, specifically RNA-sequencing.
  • Exploration of analytical challenges in antisense transcriptome investigation.

Main Results:

  • ncNATs play a role in tumorigenesis and oncogenic progression.
  • RNA-sequencing offers powerful tools for analyzing the antisense transcriptome.
  • Significant analytical challenges impede comprehensive ncNAT characterization.

Conclusions:

  • ncNATs represent a promising area for cancer biomarker discovery.
  • Targeting ncNATs may offer novel therapeutic strategies for cancer treatment.
  • Further research is needed to overcome analytical hurdles and fully elucidate ncNAT functions in cancer.