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Updated: Oct 25, 2025

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
Published on: February 23, 2018
Evolution of Plant Virus Diagnostics Used in Australian Post Entry Quarantine
Mark Whattam1, Adrian Dinsdale1, Candace E Elliott1
1Department of Agriculture, Water and Environment, 135 Donnybrook Rd, Mickleham, VIC 3156, Australia.
Abstract:
As part of a special edition for MDPI on plant virology in Australia, this review provides a brief high-level overview on the evolution of diagnostic techniques used in Australian government Post-Entry Quarantine (PEQ) facilities for testing imported plants for viruses. A comprehensive range of traditional and modern diagnostic approaches have historically been employed in PEQ facilities using bioassays, serological, and molecular techniques. Whilst these techniques have been effective, they are time consuming, resource intensive and expensive. The review highlights the importance of ensuring the best available science and diagnostic developments are constantly tested, evaluated, and implemented by regulators to ensure primary producers have rapid and safe access to new genetics to remain productive, sustainable and competitive.
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