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Related Experiment Videos

Nucleotide (cDNA) sequence encoding the horse gonadotrophin alpha-subunit.

F Stewart1, J A Thomson, S E Leigh

  • 1Thoroughbred Breeders' Association Equine Fertility Unit, Animal Research Station, Cambridge.

The Journal of Endocrinology
|November 1, 1987
PubMed
Summary

Researchers identified identical alpha-subunit gene sequences in horse pituitary and placental tissues, suggesting a single gene expresses this subunit for all equine glycoprotein hormones. This finding clarifies previous discrepancies in hormone sequencing.

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Area of Science:

  • Reproductive Biology
  • Molecular Endocrinology
  • Comparative Genomics

Background:

  • Glycoprotein hormones like FSH, LH, and CG are crucial for reproduction.
  • The alpha-subunit is a common component of these hormones, but its genetic basis in horses, particularly between pituitary and placental sources, was unclear.

Purpose of the Study:

  • To isolate and sequence cDNA clones for the horse alpha-subunit of gonadotrophic hormones.
  • To determine if the same alpha-subunit gene is expressed in both pituitary and placental tissues in horses.
  • To compare the deduced amino acid sequence with other species and investigate potential links to receptor-binding properties.

Main Methods:

  • Purification of polyadenylated mRNA from horse pituitary and placental tissues.
  • In-vitro translation and Northern hybridization using human and ovine gonadotrophin cDNA probes.

Related Experiment Videos

  • Construction of complementary DNA libraries and isolation of horse alpha-subunit cDNA clones using a human CG alpha-subunit probe.

    • Main Results:

    • Identical alpha-subunit nucleotide sequences were found in both pituitary and placental mRNA.
    • The results indicate that a single alpha-subunit gene is expressed in both horse pituitary and placenta.
    • Deduced amino acid sequence showed significant differences compared to other species, potentially explaining unique equine gonadotrophin receptor-binding properties.

    Conclusions:

    • A single gene likely encodes the alpha-subunit for all equine glycoprotein hormones (FSH, LH, CG).
    • Previous discrepancies in reported amino acid sequences for horse CG and FSH alpha-subunits may be due to sequencing errors.
    • The unique features of the horse alpha-subunit sequence may underlie the distinct receptor-binding characteristics of equine gonadotrophins.