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Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
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Constitutive cell surface expression of ZZ domain for the easy preparation of yeast-based immunosorbents
Kohei Katsurada1,2, Masahiro Tominaga1, Misato Kaishima3
1Graduate School of Science, Technology and Innovation, Kobe University.
The Journal of General and Applied Microbiology
|August 10, 2021
Abstract:
We describe a novel expression cassette that enables efficient and constitutive expression of the ZZ domain derived from Staphylococcus aureus protein A on the yeast cell surface to easily prepare yeast-based immunosorbents. Using this expression cassette containing the PGK1 promoter, a secretion signal derived from α-factor, and a Flo1-derived anchor protein, we successfully created a yeast-based immunosorbent for human serum albumin.

