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MicroRNA-449a inhibits cell proliferation and migration by regulating mutant p53 in MDA-MB-468 cells

Guangcheng Huang1, Xiaowu Zhong1,2,3, Lihua Yao1

  • 1Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.

Experimental and Therapeutic Medicine
|August 10, 2021
PubMed

Insights

MicroRNA-449a suppresses breast cancer cell growth by reducing mutant p53. This finding offers potential therapeutic strategies for triple-negative breast cancer patients with mutant p53.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple-negative breast cancer (TNBC) often harbors mutant p53, contributing to aggressive tumor behavior.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • The specific role of miR-449a in TNBC, particularly its interaction with mutant p53, requires further elucidation.

Purpose of the Study:

  • To investigate the function of microRNA (miR)-449a in the proliferation, migration, and apoptosis of MDA-MB-468 breast cancer cells.
  • To examine the relationship between miR-449a and mutant p53 in these cells.
  • To explore the potential therapeutic implications of miR-449a in TNBC.

Main Methods:

  • Cell proliferation assessed via crystal violet staining.
  • Cell migration and invasion evaluated using wound healing scratch and Transwell assays.
  • Expression levels of miR-449a and p53 determined by reverse transcription-quantitative PCR and western blotting.

Main Results:

  • Knockdown of mutant p53 inhibited proliferation and migration of MDA-MB-468 cells by suppressing the PI3K/AKT/mTOR pathway.
  • miR-449a suppressed proliferation and migration by downregulating mutant p53 expression in MDA-MB-468 cells.
  • miR-449a demonstrated tumor suppressor activity in breast cancer cells via p53 regulation.

Conclusions:

  • miR-449a acts as a tumor suppressor in breast cancer, potentially by regulating mutant p53.
  • Targeting miR-449a may offer a novel therapeutic approach for TNBC patients with mutant p53.
  • Further research into the miR-449a/p53 axis is warranted for clinical applications in breast cancer treatment.
Keywords:
breast cancerdownregulationmicroRNA-449amutant p53triple-negative breast cancer

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