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Updated: Oct 25, 2025

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Published on: January 5, 2017
Crystalline sponge X-ray analysis coupled with supercritical fluid chromatography: a novel analytical platform for
Yoshimasa Taniguchi1, Mayuka Miwa, Naoki Kitada
1Kirin Central Research Institute, Research & Development Division, Kirin Holdings Company Ltd., 26-1, Muraoka-Higashi 2-chome, Fujisawa, Kanagawa 251-8555, Japan. Yoshimasa_Taniguchi@kirin.co.jp.
Abstract:
Crystalline sponge (CS) based X-ray diffraction (XRD) analysis allows for the observation of the structure of an analyte, including its absolute configuration. Herein we report a powerful analytical platform for the separation, isolation, and structural elucidation of a target analyte in a seamless way by coupling supercritical fluid chromatography (SFC) with CS-based XRD analysis (SFC-CSXRD). The efficacy of this methodology is demonstrated by the rapid characterization of regio- and stereoisomers using three types of CSs with differing tolerances to the solvents used in SFC and guest-soaking.
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