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Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea Camellia Sinensis L. Leaves
Published on: June 15, 2019
Responses of tea plants (Camellia sinensis) with different low-nitrogen tolerances during recovery from nitrogen
Li Ruan1, Kang Wei1, Jianwu Li2
1National Center for Tea Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou, China.
Background:
Tea plants have high nitrogen (N) consumptions, whereas molecular and physiological responses of tea plants to N recovery are still unclear.
Results:
By using non-invasive micro-test technology (NMT), 15 N tracer technique, ultra-performance liquid chromatography (UPLC), and transcriptome sequencing technology, we investigated the N recovery-induced changes in N absorptions, N tissue distributions, contents of free amino acids (FAAs), and global transcription of the low-N tolerant and intolerant tea genotypes [i.e. Wuniuzao (W) and Longjing43 (L)]. The results showed that the phenotype of Wuniuzao was better than that of Longjing43 under low-N condition. The N absorption and utilization of Wuniuzao were superior to Longjing43 under N recovery. The γ-aminobutyric acid (GABA) ratio (N recovery/N deficiency) in the root of Wuniuzao was significantly higher than that of Longjing43, while the glutamic acid ratio in the root of Wuniuzao was significantly lower than that of Longjing43. This findings suggested that Wuniuzao tended to enhance the GABA synthesis, while Longjing43 tended to inhibit the GABA synthesis under N recovery. The key genes in response to N recovery in Wuniuzao included N transport (AMT and NRT), N transformation (NR, NirA, and GAD), and amino acid transport (GAT) genes. In addition, some ribosome and flavonoid biosynthesis genes might help to maintain proteome homeostasis.
Conclusion:
The N absorption and transport, and the conversion abilities of key amino acids (Glu and GABA) might improve the adaptability of tea plants to N recovery, which provided a basis for the breeding of N efficient tea varieties. © 2021 Society of Chemical Industry.
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