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Nerve growth factor immunoreactivity in mouse kidney: an immunoelectron microscopic study.
L Barajas1, E C Salido, N P Laborde
1Department of Pathology, Harbor-UCLA Medical Center, Torrance 90509.
Journal of Neuroscience Research
|January 1, 1987
Summary
This study reveals nerve growth factor (NGF) is ultrastructurally localized in mouse kidney connecting tubule cells. NGF immunoreactivity was observed in the Golgi complex, vesicles, rough endoplasmic reticulum, and polyribosomes within these cells.
Area of Science:
- Nephrology
- Cell Biology
- Molecular Biology
Background:
- Nerve growth factor (NGF) plays crucial roles in neuronal development and function.
- Previous studies have reported NGF immunolocalization in the mouse kidney using light microscopy.
Purpose of the Study:
- To investigate the ultrastructural localization of NGF within the mouse kidney.
- To identify specific cellular compartments and cell types associated with NGF in the kidney.
Main Methods:
- Utilized the pre-embedding immunoperoxidase method for electron microscopy.
- Examined NGF immunoreactivity at the ultrastructural level in mouse kidney tissue.
Main Results:
- NGF immunoreactivity was detected in connecting tubule cells of the distal nephron.
- Immunostaining for NGF was associated with the Golgi complex, vesicles, rough endoplasmic reticulum (RER), and polyribosomes.
- Intercalated cells within the kidney showed a lack of NGF immunoreactivity.
Conclusions:
- NGF is ultrastructurally localized in specific intracellular compartments of connecting tubule cells in the mouse kidney.
- These findings provide insights into the cellular mechanisms and potential functions of NGF within the renal system.